Laboratory of Molecular Radiobiology

  • Lethal and mutagenic effects of reactive oxygen species;
  • DNA repair systems in bacteria and yeast;
  • Repair of damage caused by chemotherapy in bacterial DNA;
  • Effects of UVB radiation in bacteria.

CELLULAR RESPONSE TO INJURY INDUCED BY PHYSICAL AND CHEMICAL AGENTS

    We studied the mechanisms by which cells respond to the surrounding environment. Sãoestudados repair mechanisms of the lesions produced by ionizing and non-ionizing, as well as various chemicals, mainly reactive oxygen species. Are also studied the mechanisms that lead to mutagenesis, as well as products are tested widely consumed in Brazil, in order to detect mutagenic or antimutagenic properties and therefore its potential carcinogenic or anticarcinogenic.

  1. Lethal and mutagenic effects of reactive oxygen species. The active oxygen species are implicated in the generation of a number of human diseases as well as aging. We are trying to identify the types of lesions produced in bacterial DNA by hydrogen peroxide and other reactive oxygen species, as well as the mechanisms by which cells can repair such injuries. For this study, are being used and sensitive bacterial mutants resistant to hydrogen peroxide, in addition to viruses and bacterial plasmids.
  2. Study of repair of adducts formed by binding of chemotherapeutic DNA of bacteria. The mechanism of UvrABC Nucleotide Excision has been studied on its way to repair the damage caused by agents qulrníoterápicos in the DNA of E. coli. DNA repair mechanisms have been included as part of, response to quimioyerápico in tumor cells, constituting an important system for removal of these lesions. However, the cellular response to such agents, has not been adequately characterized in the bacterium E. coli, which was proposed in our project as a model organism to study the responses expressed and the manner of its regulation.
  3. Evaluation of spontaneous and induced mutagenesis by oxidative agents in strains of Saccharomyces cerevisiae deficient in repairing bases. Little is known about the repair of oxidative damage in yeast, due to the lack of mutants sensitive to these agents. Recently, we found two genes that apparently are involved in the repair of such injuries and we are studying the biological consequences of inactivation of these genes (and Ntg1 Ntg2. S. cerevisiae). The goal is to characterize quantitatively and qualitatively spontaneous mutations and induced after treatment with oxidizing agents, using systems-bridge vectors.
 

Staff

Alvaro Augusto Costa Leitão - Head of the Laboratory

Rodrigo Soares Fortunato - Adjunct professor

Post-doctoral fellows

Tatiana Amorim Muniz de Alencar

PhD students

Tula Celeste Wilmart Gonçalves

Gabriela Silva de Almeida

Davi da Silva Barbirato

Marina das Neves Gomes

Stephan Pinheiro Frankenfeld

Igor Cabral Coutinho do Rêgo Monteiro

Master students

Juliana Patrão de Paiva

Janine Simas Cardoso Rurr

Fábio Hecht Castro Medeiros

Carolina Fittipaldi Pessôa

Undergraduate students

Bruna Alves Metzker

Jéssica Reis Bernardes

Caroline Coelho de Faria

Larissa de Souza Almeida

João Oliveira Silva

Carlos Eduardo Matos de Lima

Technicians

Leonardo da Silva Vidal (NS)

Janine Simas Cardoso Rurr (NS)

Rita de Cássia de Albuquerque (NM)

 

Programa: 
Structural and Molecular Biology
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